2009-03-24

[文摘] 人類幹細胞轉殖使小鼠重見光明

SkyOrggLee 報導

華聖頓大學生物結構研究所最新研究發現,利用人類胚胎幹細胞轉殖在小鼠眼球,可以再生感光細胞,使小鼠重見光明。

常見失明的原因的原因是由眼睛中的感光細胞(photoreceptors)死亡所造成,華聖頓大學生物結構所(Department of Biological Structure, University of Washington)RA. Thomas教授所帶領的研究團隊,將人類胚胎幹細胞(human Embryonic Stem Cell)轉殖在小鼠眼球中,成功地將幹細胞分化成感光細胞,並插入在小鼠眼內的感光細胞層,經過初步的證實,此分化的細胞具有感光的功能

Thomas的研究團隊將人類胚胎幹細胞轉殖在成熟的Crx缺失的小鼠,以視網膜下腔注射的方式,經過一至六週後觀察,可找到由人類幹細胞發育而成的感光細胞,並利用electroretinographic (ERG)的方式檢測,證實這些新生的感光細胞也具有感光能力。

文章摘要如下: from NCBI

 Cell Stem Cell. 2009 Jan 9;4(1):73-9.Links

Transplantation of human embryonic stem cell-derived photoreceptors restores some visual function in Crx-deficient mice.

Department of Biological Structure, University of Washington, Seattle, WA 98195, USA.

Some of the most common causes of blindness involve the degeneration of photoreceptors in the neural retina; photoreceptor replacement therapy might restore some vision in these individuals. Embryonic stem cells (ESCs) could, in principle, provide a source of photoreceptors to repair the retina. We have previously shown that retinal progenitors can be efficiently derived from human ESCs. We now show that retinal cells derived from human ESCs will migrate into mouse retinas following intraocular injection, settle into the appropriate layers, and express markers for differentiated cells, including both rod and cone photoreceptor cells. After transplantation of the cells into the subretinal space of adult Crx(-/-) mice (a model of Leber's Congenital Amaurosis), the hESC-derived retinal cells differentiate into functional photoreceptors and restore light responses to the animals. These results demonstrate that hESCs can, in principle, be used for photoreceptor replacement therapies.

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